Question 1

Compare and contrast recombinant DNA and gene therapy.

Accepted Answer

Recombinant DNA and gene therapy are bot...

Question 2

Which PCR step synthesizes complementary DNA strands?

Accepted Answer

A)   Add DNA polymerase and nucleotides at 72°C.
B)   Cool DNA to between 50°C and 65°C.
C)   Add primers.
D)   Heat target DNA to 94°C.
E)   Repeat the cycle of heating and cooling.F) C) and D)
G) A) and D)

Question 3

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

The answer of Genomic _____ are collections of isolated genes...

Question 4

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

Accepted Answer

A)   the amino acids at the amino terminus of the Cas nuclease
B)   the guide RNA crRN
A)   carried by the Cas nuclease
C)   the amino acids at the carboxyl terminus of the Cas nuclease
D)   an antibody-like region of the Cas nucleaseE) B) and C)
F) None of the above

Question 6

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Accepted Answer

*The DNA profile of each individual is h...

Question 7

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

Accepted Answer

A)   cloning
B)   gene therapy
C)   antisense therapeutic
D)   DNA fingerprintingE) A) and C)
F) A) and D)

Question 8

EcoRI and HindIII are

Accepted Answer

A)   palindromes.
B)   reverse transcriptases.
C)   restriction endonucleases.
D)   ligases.
E)   DNA polymerases.F) A) and D)
G) A) and E)

Question 9

Which type of DNA analysis is likely to be most effective when analyzing the ancient remains of a mummified body?

Accepted Answer

A)   whole genome sequencing
B)   analysis of short tandem repeats
C)   analysis of cDNA
D)   analysis of mitochondrial DNAE) A) and B)
F) A) and C)

Question 10

Which of the following is a serious concern when performing PCR?

Accepted Answer

A)   The high temperature needed may denature the DNA.
B)   Restriction enzymes are difficult to obtain in adequate quantities.
C)   Contaminating DNA may be introduced and amplified.
D)   Lab personnel may be exposed to radiation.
E)   PCR is a very time-consuming process.F) C) and D)
G) C) and E)

Question 11

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)   used as cloning vectors.
B)   sources of heat stable DNA polymerases.
C)   genetically engineered bacteria.
D)   principal sources of restriction endonucleases.E) B) and C)
F) C) and D)

Question 12

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Accepted Answer

A)   genetic engineering.
B)   biotechnology.
C)   recombinant DNA technology.
D)   gel electrophoresis.
E)   gene probes.F) B) and D)
G) A) and E)

Question 13

When DNA is heated to just below boiling 90°C-95°C) ,

Accepted Answer

A)   the sugar phosphate backbone of the DNA strands breaks.
B)   the sugar, phosphate, and base come apart in each nucleotide.
C)   the helix unwinds but hydrogen bonds between the bases remain intact.
D)   the two DNA strands separate completely.
E)   nothing happens until the boiling point is reached 100o
C)  .F) B) and E)
G) A) and E)

Question 14

Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using

Accepted Answer

A)   palindromes.
B)   reverse transcriptases.
C)   restriction endonucleases.
D)   ligases.
E)   DNA polymerases.F) A) and E)
G) A) and D)

Question 15

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Accepted Answer

A)   genetic engineering.
B)   biotechnology.
C)   recombinant DNA.
D)   gel electrophoresis.
E)   gene probes.F) All of the above
G) A) and B)

Question 16

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Accepted Answer

A)   DNA fingerprinting
B)   microarray analysis
C)   gene mapping
D)   Western blot
E)   antisense therapyF) A) and E)
G) D) and E)

Question 17

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Accepted Answer

A)   mitochondrial DNA
B)   chloroplast DNA
C)   rRNA
D)   mRNA
E)   tRNAF) None of the above
G) A) and B)

Question 19

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects.

Accepted Answer

A)   Escherichia coli
B)   Saccharomyces cerevisiae
C)   Thermus aquaticus
D)   Pseudomonas syringae
E)   Pseudomonas fluorescensF) None of the above
G) B) and D)

Question 20

DNA polymerases used in PCR

Accepted Answer

A)   use an RNA template to make complementary DNA.
B)   must remain active at very cold temperatures.
C)   include Taq polymerases and Vent polymerase.
D)   are labeled with fluorescent dyes.
E)   All of these choices are correct.F) A) and E)
G) C) and D)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Which type of DNA analysis is likely to be most effective when analyzing the ancient remains of a mummified body?

Correct AnswerverifiedShow Answer

Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using

Correct AnswerverifiedShow Answer

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

Correct AnswerverifiedShow Answer

Transformation and transfection are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Correct Answerverified*The DNA profile of each individual is h...View AnswerShow Answer

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

Correct AnswerverifiedShow Answer

When DNA is heated to just below boiling 90°C-95°C) ,

Correct AnswerverifiedShow Answer

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR

Correct AnswerverifiedShow Answer

Compare and contrast recombinant DNA and gene therapy.

Correct AnswerAnswered by ExamLex AIRecombinant DNA and gene therapy are bot...View AnswerShow Answer

Which of the following is a serious concern when performing PCR?

Correct AnswerverifiedShow Answer

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Correct AnswerverifiedShow Answer

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects.

Correct AnswerverifiedShow Answer

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complementary DNA strands?

Correct AnswerverifiedShow Answer

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Correct AnswerverifiedShow Answer

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*The DNA profile of each individual is h...
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Recombinant DNA and gene therapy are bot...
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